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High-Throughput Multi-Sample Sonicator

Stop sonicating one sample at a time. Process all 96 in one run.

PIXUL is the only sonicator that processes 96 samples at once, truly in parallel, not sequentially. It shears chromatin, DNA, RNA, and protein in a standard $2 microplate in 10 to 30 minutes, with identical results in every well.

Every sample. Every well. Every time.

96
Samples processed simultaneously
42+
Peer-reviewed publications
95+
Instruments installed worldwide
$2
Per plate, standard consumables

Life science experiments scaled. Mechanical shearing hasn't.

Life science research is scaling: larger cohorts, more conditions, multi-omics, precision medicine. But the foundational step, mechanical shearing, hasn't evolved in decades.

Probe sonicators One sample at a time. Operator-dependent results. Localized thermal spikes that degrade sensitive protein-DNA interactions. Cross-contamination risk between samples.
Water bath sonicators 6 to 12 samples per run. Multi-hour cycling times to reach target fragment sizes. Physical geometry limits true 96-well processing.
Focused ultrasonicators Process wells sequentially, taking over 7 hours for 96 samples. Proprietary consumables at ~$400 per plate. Vendor lock-in.
Enzymatic methods Sequence and GC-content bias. Limited to specific epigenetics applications. Not applicable for gDNA, RNA, proteomics, FFPE, or cfDNA.

The ideal solution would process all your samples at once, 96 in parallel, not sequentially. It would deliver identical shearing in every well, validated in peer-reviewed publications. It would use standard consumables, not proprietary ones at $400 per plate. And it would work across all your applications (chromatin, DNA, RNA, protein, FFPE) so you invest in one instrument, not three.

Built to solve three problems at once

PIXUL was engineered by researchers at the University of Washington who needed throughput, consistency, and freedom from proprietary consumables in one instrument.

PIXUL processing 96 samples simultaneously in a standard microplate

96 samples in 10 to 30 minutes vs. 7+ hours

PIXUL's arrayed transducers shear your entire experiment simultaneously. Culture, fix, and sonicate in the same plate with no sample transfers and no waiting.

Focused ultrasonicator: ~35 min/sample = 7+ hours for 96. PIXUL: 96 in one run.
307 ± 35 bp
across all 96 wells
272
342

307 ± 35 bp across all 96 wells (NAR 2019)

2 MHz megasonication delivers uniform cavitation with no hot spots and no cold spots. Tighter fragment distributions than focused ultrasonicators in published head-to-head comparisons.

Chromatin: 440 ± 53 bp (PIXUL) vs. 532 ± 77 bp (focused ultrasonicator tubes)
Standard $2 microplate next to $400 proprietary consumable, illustrating PIXUL cost savings

$2 per plate vs. ~$400 proprietary consumables

Standard round-bottom 96-well plates. No vendor lock-in. A busy lab running 3 plates per week saves over $60,000 per year in consumables alone, more than the cost of the instrument.

Break-even on consumable savings in under 12 months for a 3-plate/week lab.

Chromatin. DNA. RNA. Protein. FFPE. cfDNA. One instrument.

PIXUL is the upstream sample preparation engine for your entire lab. Validated across six application areas with peer-reviewed publications and direct integration with Active Motif's downstream kits.

MultiomicsTracks96 (2024): 8-dimensional omics from frozen + FFPE tissues on a single PIXUL-prepared plate.
Chromatin / ChIP-Seq Genomic DNA / WGS Proteomics / LC-MS/MS RNA Processing FFPE Tissues cfDNA / Liquid Biopsy
PIXUL supporting multiple application workflows from chromatin to proteomics

How PIXUL compares

An honest comparison across the four major approaches to mechanical shearing. Data sourced from peer-reviewed publications and manufacturer specifications.

Feature PIXUL Probe Sonicators Water Bath Sonicators Focused Ultrasonicators
Throughput 96 samples simultaneously 1 sample at a time 6–12 samples per run Sequential (7+ hrs for 96)
Consumable Cost ~$2 per plate (standard) ~$0.10 per tube ~$5–10 per batch ~$400 per plate (proprietary)
Fragment Consistency 307 ± 35 bp (all 96 wells) Operator-dependent Good (within 6–12 samples) 532 ± 77 bp (tubes)
Vendor Lock-in None, standard plates None Moderate High ($400 proprietary plates)
Walk-Away Operation Yes, touchscreen, unattended No, manual, hands-on Partial Yes
Application Range 6+ (chromatin, DNA, RNA, protein, FFPE, cfDNA) Limited Primarily chromatin Broad

What scientists are saying

I'm pleased to report that the PIXUL system has been outstanding in our hands. We've seen much better resolution, an increased number of differentially regulated genomic regions, and highly reproducible results.

Dr. Isidoro Cobo University of Alabama at Birmingham

Since the first time I sonicated my samples and got expected results in ~30 min, I don't want to look back at those days when I had to hold my tube and be seated for hours sonicating all my samples one by one.

PIXUL User Life sciences researcher
Nucleic Acids Research Peer-reviewed validation (2019)
42+ Publications Cited across disciplines
95+ Labs Worldwide Including pharma repeat purchases
AACR, ASHG, AAI 2024 Conference poster presentations

Built by researchers who couldn't find what they needed. Dr. Karol Bomsztyk at the University of Washington had developed Matrix-ChIP to accelerate chromatin immunoprecipitation, but sonication remained the bottleneck. Teaming up with ultrasound physicist Dr. Tom Matula, they engineered a new class of instrument: arrayed 2 MHz transducers that shear all 96 wells simultaneously. Active Motif licensed the technology for global commercialization.

Ready to process 96 samples at once?

Request a personalized quote or try PIXUL with your own samples through our remote evaluation program.

Common questions about PIXUL

What is the PIXUL Multi-Sample Sonicator?
PIXUL is a high-throughput multi-sample sonicator from Active Motif that uses arrayed 2 MHz megasonication transducers to shear up to 96 samples simultaneously in standard microplates. It processes chromatin, DNA, RNA, and protein in 10 to 30 minutes per run, validated in Nucleic Acids Research (2019).
How many samples can PIXUL process at once?
PIXUL processes up to 96 samples truly in parallel, not sequentially. Each column of the 96-well plate has its own dedicated pair of acoustic transducers, delivering identical shearing to every well simultaneously. A full plate completes in 10 to 30 minutes depending on the application and target fragment size. You can run any number from 1 to 96.
What applications does PIXUL support?
PIXUL is validated for chromatin shearing (ChIP-Seq, CUT&Tag, ATAC-Seq), genomic DNA fragmentation (WGS, NGS library prep), protein extraction for mass spectrometry (integrated with S-Trap for LC-MS/MS), RNA processing, FFPE tissue homogenization, and cell-free DNA processing from clinical specimens including liquid biopsies. The 2024 MultiomicsTracks96 publication demonstrated 8-dimensional omics from frozen and FFPE tissues on a single plate.
How does PIXUL compare to focused ultrasonicators?
PIXUL processes all 96 samples simultaneously in 10 to 30 minutes, while focused ultrasonicators process wells sequentially, taking over 7 hours for a full 96-well plate. PIXUL uses standard $2 plates versus ~$400 proprietary consumables, saving a busy lab over $60,000 per year. Published data in Nucleic Acids Research showed PIXUL achieved tighter fragment distributions (440 ± 53 bp vs. 532 ± 77 bp for focused ultrasonicator tubes) with lower ChIP background noise, using only one-third the starting material.